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p srcy416  (ABclonal Biotechnology)


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    Structured Review

    ABclonal Biotechnology p srcy416
    P Srcy416, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti-phospho-src+(ap0511)/Phospho-SRC+Family-Y416+Rabbit+pAb/pmc13126418-38-46-86
    Average 94 stars, based on 12 article reviews
    p srcy416 - by Bioz Stars, 2026-11
    94/100 stars

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    Related Articles

    Western Blot:

    Article Title: Loss of Myo19 increases metastasis by enhancing microenvironmental ROS gradient and chemotaxis
    Article Snippet: Cells were transfected with 2 μg plasmid DNA in opti-MEM (Invitrogen) containing 2 μL NeofectTM DNA transfection reagent (TF20121201) following the protocol for 24–48 h. .. The following antibodies were used in this study: rabbit anti-Myo19 (HPA059715, 1:1000 for western blotting and 1:800 for IHC) and mouse anti-α-tubulin (T9026, 1:4000 for western blotting) from Sigma-Aldrich; rabbit anti-Mic60 (A2751, 1:1000 for western blotting), rabbit anti-Src ( A19119 , 1:1000 for western blotting) rabbit anti- pSrc Y416 (AP0452, 1:1000 for western blotting) from ABclonal; mouse anti-RhoA (ARH04, 1:800 for western blotting) from Cytoskeleton; rabbit anti-GAPDH (ab181602, 1:4000 for western blotting) from Abcam; mouse anti-Nrf2 (sc-365949, 1:800 for western blotting) from Santa Cruz; anti-mouse (sc-516102, 1:4000) and anti-rabbit (sc-2004, 1:4000) horseradish peroxidase (HRP)-conjugated secondary antibodies from Santa Cruz Biotechnology. .. For reagents, CellTrackerTM Red CMTPX ( C34552 ), CellTrackerTM Green CMFDA (C2925) and MitoSOX ( M36008 ) were purchased from ThermoFisher Scientific.

    Immunohistochemistry:

    Article Title: Loss of Myo19 increases metastasis by enhancing microenvironmental ROS gradient and chemotaxis
    Article Snippet: Cells were transfected with 2 μg plasmid DNA in opti-MEM (Invitrogen) containing 2 μL NeofectTM DNA transfection reagent (TF20121201) following the protocol for 24–48 h. .. The following antibodies were used in this study: rabbit anti-Myo19 (HPA059715, 1:1000 for western blotting and 1:800 for IHC) and mouse anti-α-tubulin (T9026, 1:4000 for western blotting) from Sigma-Aldrich; rabbit anti-Mic60 (A2751, 1:1000 for western blotting), rabbit anti-Src ( A19119 , 1:1000 for western blotting) rabbit anti- pSrc Y416 (AP0452, 1:1000 for western blotting) from ABclonal; mouse anti-RhoA (ARH04, 1:800 for western blotting) from Cytoskeleton; rabbit anti-GAPDH (ab181602, 1:4000 for western blotting) from Abcam; mouse anti-Nrf2 (sc-365949, 1:800 for western blotting) from Santa Cruz; anti-mouse (sc-516102, 1:4000) and anti-rabbit (sc-2004, 1:4000) horseradish peroxidase (HRP)-conjugated secondary antibodies from Santa Cruz Biotechnology. .. For reagents, CellTrackerTM Red CMTPX ( C34552 ), CellTrackerTM Green CMFDA (C2925) and MitoSOX ( M36008 ) were purchased from ThermoFisher Scientific.

    other:

    Article Title: Ouabain Enhances Cell-Cell Adhesion Mediated by β 1 Subunits of the Na + ,K + -ATPase in CHO Fibroblasts
    Article Snippet: Rabbit anti-phospho-Akt (Ser473, D9E), rabbit anti-phospho-p44/42 MAPK (Erk1/2, Thr202/Tyr204, D13.14.4E); rabbit anti-phospho-Src Family (Tyr416, D49G4); rabbit anti-Akt (pan, C67E7); rabbit anti-p44/42 MAPK (Erk1/2, 137F5), and rabbit anti-Src (36D10) (4060, 4370, 6943, 4691, 4695, and 2109, respectively, Cell Signaling Technologies) The following rabbit antibodies were obtained from Abclonal (Woburn, MA, USA): anti-Src total (A0324), anti-p120 catenin (A1641), anti-β catenin (A11343), anti-E cadherin (A3044), anti-phospho-Src-Y416 (AP0480), anti-ERK1/ERK2 total (A16686), anti-phospho-ERK1-T202/Y204 and ERK2-T185/Y187 (AP0472).

    Article Title: Src‐mediated phosphorylation of GAPDH regulates its nuclear localization and cellular response to DNA damage
    Article Snippet: Anti-Src (A11707), anti-p-SrcY419 (AP0452), anti-p-Src-Y530 (AP0185), and cleaved (active)-Caspase 3 (A11021) antibodies were purchased from ABclonal.

    Article Title: TNFα modulates PANX1 activation to promote ATP release and enhance P2RX7-mediated antitumor immune responses after chemotherapy in colorectal cancer.
    Article Snippet: The antibodies used were as follows: anti-TNFR1 (A12540, ABclonal), anti-TNFR2 (A12536, ABclonal), anti-caspase-3 (#9662, Cell Signaling Tech. and IR96-401, iREAL Bio.), anti-cleaved caspase-3 (#9661 Cell Signaling Tech.), anti-PANX1 (ab233479, Abcam and #91137, Cell Signaling Tech.), anti-caspase-8 (SC-81656, Santa Cruz), anti-phospho-Src family kinase (SFKY416, #2101, Cell Signaling Tech. and AP0511, ABclonal), anti-RIPK1 (#4926, Cell Signaling Tech.), anti-RIPK3 (#10188, Cell Signaling Tech.), anti-MLKL (#14993, Cell Signaling Tech.) and anti-phospho-MLKLS358 (#91689, Cell Signaling Tech.) antibodies.

    Incubation:

    Article Title: Panaxadiol inhibits synaptic dysfunction in Alzheimer's disease and targets the Fyn protein in APP/PS1 mice and APP-SH-SY5Y cells.
    Article Snippet: Panaxadiol inhibits synaptic dysfunction in Alzheimer's disease and targets the Fyn protein in APP/PS1 mice and APP-SH-SY5Y cells

    Article Title: Bioinformatics study of the potential therapeutic effects of ginsenoside Rh3 in reversing insulin resistance
    Article Snippet: .. The membranes were incubated with the following primary antibodies: Anti-Phospho-EGFR antibody (GB114199-100), Anti-ESR1 antibody (GB111843-100) and Anti-MAPK1 antibody (GB11370-100) were purchased from Servicebio Technology (Wuhan, China); Anti-Phospho-SRC (AP0511) and Anti-Hsp90a antibody (A13501) were purchased from ABclonal (Wuhan, China). ..



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